Substrate Inhibition Kinetics of Phenol Degradation by Pseudomonas Aeruginosa (ncim 2074)

The biodegradation of phenol by Pseudomonas aeruginosa (NCIM 2074) was carried out in a batch system and the optimum conditions were determined. At an optimum pH 7, temperature 32°C, agitation speed 160 rpm, carbon source (glucose) 0.5 g/l, nitrogen source (ammonium chloride) 0.2 g/l, metal ion concentration (Zn) 0.02 g/l the percentage of phenol degradation was 80.45%. Phenol had a strong inhibitor effect on the microbial growth and Haldane model was used to describe the substrate inhibition. Model parameters were estimated by non-linear regression analysis using Force 2.0 software. The values of the parameters obtained were μ max = 0.306 h; K S = 108.87 mg/l and K I = 567.84 mg/l.